{"id":1411,"date":"2019-12-06T08:52:44","date_gmt":"2019-12-06T06:52:44","guid":{"rendered":"http:\/\/titan.physx.u-szeged.hu\/~adoptim\/?p=1411"},"modified":"2020-08-27T15:11:27","modified_gmt":"2020-08-27T13:11:27","slug":"nanoscale-reconstruction-of-sarcomeres","status":"publish","type":"post","link":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/?p=1411","title":{"rendered":"Nanoscale reconstruction of sarcomeres"},"content":{"rendered":"\n<p class=\"has-text-align-justify wp-block-paragraph\">Sarcomeres are extremely highly ordered macromolecular assemblies where  structural organization is intimately linked to their functionality as  contractile units. Although the structural basis of actin and Myosin  interaction is revealed at a quasiatomic resolution, much less is known  about the molecular organization of the I-band and H-zone. We report the  development of a powerful nanoscopic approach, combined with a  structure-averaging algorithm, that allowed us to determine the position  of 27 sarcomeric proteins in <em>Drosophila melanogaster<\/em> flight  muscles with a quasimolecular, \u223c5- to 10-nm localization precision. With  this protein localization atlas and template-based protein structure  modeling, we have assembled refined I-band and H-zone models with  unparalleled scope and resolution. In addition, we found that actin  regulatory proteins of the H-zone are organized into two distinct  layers, suggesting that the major place of thin filament assembly is an  M-line\u2013centered narrow domain where short actin oligomers can form and  subsequently anneal to the pointed end.<\/p>\n\n\n\n<span class=\"altmetric-embed\" data-hide-no-mentions=\"true\" data-badge-popover=\"right\" data-doi=\"10.1083\/jcb.201907026\"><\/span><span class=\"__dimensions_badge_embed__\" data-doi=\"10.1083\/jcb.201907026\" data-style=\"small_rectangle\" data-hide-zero-citations=\"true\"><\/span>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\"><p><strong>Nanoscopy reveals the layered organization of the sarcomeric H-zone and I-band complexes<\/strong> &#8211;  Szil\u00e1rd Szikora, Tam\u00e1s Gajdos, Tibor Nov\u00e1k, D\u00e1vid Farkas, Istv\u00e1n F\u00f6ldi, Peter Lenart, Mikl\u00f3s Erd\u00e9lyi, J\u00f3zsef Mih\u00e1ly<\/p><p><a href=\"https:\/\/t.co\/1f3ep2EoxI?amp=1\">https:\/\/rupress.org\/jcb\/article\/219\/1\/e201907026\/132617\/Nanoscopy-reveals-the-layered-organization-of-the<\/a><\/p><p> <a href=\"https:\/\/doi.org\/10.1083\/jcb.201907026\">https:\/\/doi.org\/10.1083\/jcb.201907026<\/a><\/p><\/blockquote>\n\n\n\n<figure class=\"wp-block-pullquote\"><blockquote><p>Bio-Protocol: <a rel=\"noreferrer noopener\" href=\"https:\/\/bio-protocol.org\/e3654\" target=\"_blank\">e3654<\/a><\/p><cite>(in-press: <a rel=\"noreferrer noopener\" href=\"https:\/\/bio-protocol.org\/archive.aspx?issid=1242\" target=\"_blank\">2020\/06\/20<\/a>)<\/cite><\/blockquote><\/figure>\n\n\n\n<!--more-->\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/jcb_201907026_fig1-scaled.png\"><img loading=\"lazy\" decoding=\"async\" width=\"2560\" height=\"1214\" data-attachment-id=\"1424\" data-permalink=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/?attachment_id=1424\" data-orig-file=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/jcb_201907026_fig1-scaled.png\" data-orig-size=\"2560,1214\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"jcb_201907026_fig1\" data-image-description=\"\" data-image-caption=\"\" data-large-file=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/jcb_201907026_fig1-1024x485.png\" src=\"https:\/\/i1.wp.com\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/jcb_201907026_fig1-scaled.png?fit=530%2C251\" alt=\"\" class=\"wp-image-1424\" srcset=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/jcb_201907026_fig1-scaled.png 2560w, https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/jcb_201907026_fig1-300x142.png 300w, https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/jcb_201907026_fig1-1024x485.png 1024w, https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/jcb_201907026_fig1-768x364.png 768w, https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/jcb_201907026_fig1-1536x728.png 1536w, https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/jcb_201907026_fig1-2048x971.png 2048w\" sizes=\"auto, (max-width: 2560px) 100vw, 2560px\" \/><\/a><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Sarcomeres are extremely highly ordered macromolecular assemblies where structural organization is intimately linked to their functionality as contractile units. Although the structural basis of actin and Myosin interaction is revealed<\/p>\n","protected":false},"author":1,"featured_media":1412,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[10],"tags":[23,36],"class_list":["post-1411","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publication","tag-publication","tag-sarcomere"],"jetpack_publicize_connections":[],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2019\/12\/JCB_218_continuous_default.cvf1-50-2111611155.gif","_links":{"self":[{"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/posts\/1411","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1411"}],"version-history":[{"count":19,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/posts\/1411\/revisions"}],"predecessor-version":[{"id":1591,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/posts\/1411\/revisions\/1591"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/media\/1412"}],"wp:attachment":[{"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1411"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1411"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1411"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}