{"id":1605,"date":"2020-10-26T16:24:08","date_gmt":"2020-10-26T14:24:08","guid":{"rendered":"http:\/\/titan.physx.u-szeged.hu\/~adoptim\/?p=1605"},"modified":"2020-11-04T08:26:19","modified_gmt":"2020-11-04T06:26:19","slug":"syndecan-4-modulates-cell-polarity-and-migration-by-influencing-centrosome-positioning-and-intracellular-calcium-distribution","status":"publish","type":"post","link":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/?p=1605","title":{"rendered":"Syndecan-4 Modulates Cell Polarity and Migration by Influencing Centrosome Positioning and Intracellular Calcium Distribution"},"content":{"rendered":"\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<div class=\"wp-block-group\"><div class=\"wp-block-group__inner-container is-layout-flow wp-block-group-is-layout-flow\">\n<p class=\"wp-block-paragraph\">In collaboration with the <a rel=\"noreferrer noopener\" href=\"http:\/\/www.biochem.szote.u-szeged.hu\/tartalom\/angol\/muscleadaptation_En.html\" target=\"_blank\">Muscle Adaptation Group<\/a>, we have published a new paper in Frontiers in Cell and Developmental Biology. Our join research venture studied the regeneration process in the tissue on a molecular level.<\/p>\n\n\n\n<blockquote class=\"wp-block-quote is-layout-flow wp-block-quote-is-layout-flow\"><p>Becsky, Daniel, et al. &#8220;Syndecan-4 Modulates Cell Polarity and Migration by Influencing Centrosome Positioning and Intracellular Calcium Distribution.&#8221; <em>Frontiers in Cell and Developmental Biology<\/em> (2020).<\/p><cite><a href=\"https:\/\/doi.org\/10.3389\/fcell.2020.575227\">https:\/\/doi.org\/10.3389\/fcell.2020.575227<\/a><\/cite><\/blockquote>\n\n\n\n<span class=\"altmetric-embed\" data-hide-no-mentions=\"true\" data-badge-popover=\"right\" data-badge-type=\"donut\" data-doi=\"10.3389\/fcell.2020.575227\"><\/span><span class=\"__dimensions_badge_embed__\" data-doi=\"10.3389\/fcell.2020.575227\" data-style=\"small_circle\" data-hide-zero-citations=\"true\"><\/span>\n\n\n\n<p class=\"wp-block-paragraph\">Here are some Large Images captured with our system:<\/p>\n\n\n\n<figure class=\"wp-block-gallery columns-2 is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\"><ul class=\"blocks-gallery-grid\"><li class=\"blocks-gallery-item\"><figure><img loading=\"lazy\" decoding=\"async\" width=\"13820\" height=\"8320\" data-attachment-id=\"1607\" data-permalink=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/?attachment_id=1607\" data-orig-file=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_TR30012.png\" data-orig-size=\"13820,8320\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"20190321_TR30012\" data-image-description=\"&lt;p&gt;A representativewide-field  fluorescence  microscopy  image depictsthe  actinskeletonsof scrambled myoblasts around the cell-free zone.The inserts depict the lamellipodial actin structures inthe migrating cells, whichwere imaged usingdSTORM.The cells were fixed 2h  after  confluent cultures  were  scratched,  and  the  actin  filaments  were  stained withAlexa Fluor 647-conjugated phalloidin (red). Nuclei are shown in blue (Hoechst 33258).&lt;\/p&gt;\n\" data-image-caption=\"&lt;p&gt;Actin cytoskeletal structure of scrambled myoblasts around the cell-free zone.&lt;\/p&gt;\n\" data-large-file=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_TR30012.png\" src=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_TR30012.png\" alt=\"\" data-id=\"1607\" data-full-url=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_TR30012.png\" data-link=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/?attachment_id=1607\" class=\"wp-image-1607\" srcset=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_TR30012.png 13820w, https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_TR30012-300x181.png 300w\" sizes=\"auto, (max-width: 13820px) 100vw, 13820px\" \/><figcaption class=\"blocks-gallery-item__caption\">Actin cytoskeletal structure of scrambled myoblasts around the cell-free zone.<\/figcaption><\/figure><\/li><li class=\"blocks-gallery-item\"><figure><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"605\" data-attachment-id=\"1606\" data-permalink=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/?attachment_id=1606\" data-orig-file=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_97.png\" data-orig-size=\"13720,8100\" data-comments-opened=\"1\" data-image-meta=\"{&quot;aperture&quot;:&quot;0&quot;,&quot;credit&quot;:&quot;&quot;,&quot;camera&quot;:&quot;&quot;,&quot;caption&quot;:&quot;&quot;,&quot;created_timestamp&quot;:&quot;0&quot;,&quot;copyright&quot;:&quot;&quot;,&quot;focal_length&quot;:&quot;0&quot;,&quot;iso&quot;:&quot;0&quot;,&quot;shutter_speed&quot;:&quot;0&quot;,&quot;title&quot;:&quot;&quot;,&quot;orientation&quot;:&quot;0&quot;}\" data-image-title=\"20190321_97\" data-image-description=\"&lt;p&gt;A representative wide-field fluorescence microscopy image depicts the actin skeletons of shSDC4#2 (i.e., syndecan-4 knockdown) myoblasts around the cell-free zone. The inserts depict the lamellipodial actin structures in the migrating cells, which were imaged using dSTORM. The cells were fixed 2h after confluent cultures were scratched, and the actin filaments were stained with Alexa Fluor 647-conjugated phalloidin (red). Nuclei are shown in blue (Hoechst 33258).&lt;\/p&gt;\n\" data-image-caption=\"&lt;p&gt;Actin cytoskeletal structure of shSDC4#2myoblastsaround the cell-free zone. &lt;\/p&gt;\n\" data-large-file=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_97-1024x605.png\" src=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_97-1024x605.png\" alt=\"\" data-id=\"1606\" data-full-url=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_97.png\" data-link=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/?attachment_id=1606\" class=\"wp-image-1606\" srcset=\"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_97-1024x605.png 1024w, https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_97-300x177.png 300w, https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_97-768x453.png 768w, https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_97-1536x907.png 1536w, https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/20190321_97-2048x1209.png 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><figcaption class=\"blocks-gallery-item__caption\">Actin cytoskeletal structure of shSDC4#2myoblastsaround the cell-free zone. <\/figcaption><\/figure><\/li><\/ul><\/figure>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>\n","protected":false},"excerpt":{"rendered":"<p>In collaboration with the Muscle Adaptation Group, we have published a new paper in Frontiers in Cell and Developmental Biology. Our join research venture studied the regeneration process in the<\/p>\n","protected":false},"author":1,"featured_media":1620,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[10],"tags":[],"class_list":["post-1605","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-publication"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/wp-content\/uploads\/2020\/10\/CellAdhesionandMigration.png","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/posts\/1605","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1605"}],"version-history":[{"count":13,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/posts\/1605\/revisions"}],"predecessor-version":[{"id":1623,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/posts\/1605\/revisions\/1623"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=\/wp\/v2\/media\/1620"}],"wp:attachment":[{"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1605"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1605"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/titan.physx.u-szeged.hu\/~adoptim\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1605"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}