A Matlab application for processing and reviewing localization microscopy data. (v3.0)

rainSTORM v2.37 is considered old-stable can be downloaded from the Laser Analytics Group site.

rainSTORM v3.0 (2015. Oct) is an old-stable release.

• Release highlights:
  • new Graphical User Interface
  • new Multi Channel option
  • cleaned Workspace in Matlab
  • corrected background selection and subtraction
  • new file formats (e.g. nd2)
  • new Reporting tool (save reviewed data)
  • new Save Localizations tool (in csv)
  • new Bridge Tracker Tool
  • new Channel Merge Tool
  • new batch function for automated reviewing.
• Tested with Matlab R2014a-R2016a (it might run on older versions).
  • Required Toolboxes: Image Processing, Parallel Computing
• Run with: startup; rainSTORM;
• EOL 2017.01.01. Won’t recieve any updates.

rainSTORM v3.1.8 (2020. Jan) is stable, suggested for every day use.

• 2016. May Release highlights v3.1:
  • new 2D Single-Gaussian Localization Algorithm
  • new 2D Multi-Gaussian Localization Algorithm
  • new background estimation methods (linear, constant)
  • updated Import and Export Super Resolution Parameters
  • updated Save & Load Sessions
  • new 2 Marker-Less Drift Correction Methods (Auto/Blind, BaSDI)
  • new/updated Histogram and Bridge Tracking Tools
• 2016. July updates (v3.1.1): Bridge tracker colourmap, minor filter range changes, rejected image viewing changes, experimental functions
• 2017. January updates (v3.1.3): Compatibility with Matlab R2016a & R2016b
  • R2016a/b: Fix a warning in rainSTORM_parfor_progress.
  • R2016b: Change how a colormap is applied on a super-resolved image.
• 2017. March update (v3.1.4): Quick fix for R2017a.
• 2017. December note: Works with R2018a.
• 2018. January update (v3.1.5): Read single-frame tiff files in a folder.
  • new “tiff-folder” read method is introduced to handle single-frame tiff files in a folder. When using this method, keep every measurement in separate folders, and select the first image for a measurement in the file browser.
• 2018. May update (v3.1.6): BioFormats update to 5.8.2.
  • NIS 5.0+ changes the nd2 file format a bit, thus an update is required.
• 2019. May update (v3.1.7): Export select ROI feature
  • Backport ExportTracker tool to export localization data from selected ROIs on the Super-Resolution Image. IFM Analyser requires this feature.
  • Updated Auto Drift Algorithm with comments.
  • Removed the BaSDI Drift Correction button from the GUI, as it wasn’t as good as expected.
• 2020. Jan update (v3.1.8): Bio Formats update to 6.3.1

Tested with Matlab R2014b-R2019a. Required Matlab Toolboxes:

• Required by main rainSTORM: Image Processing Toolbox
• Required by Auto Drift Correction: Optimization Toolbox
• Required to enable multi-threaded localization: Paralell Computing Toolbox

Run with: startup; rainSTORM;

Participation in SMLM Software Challenge 2016

User Guides for v3.1.*:

• rainSTORM_2016_user_guide_ver1 – User Guide version 1
• smlsm_2016_GT – poster presentation about using rainSTORM
• rainSTORM-blind-drift-correction – description of the auto drift correction algorithm

rainSTORM v3.2 is under development, and is maintained at gitlab.com. To get involved or to be an early adopter, please contact Miklós Erdélyi for access.

Note#1: we keep only major versions, and any minor release overwrites the previous one.

Note#2 (2018.01.09.): After applying the Windows 10 security fix for the Meltdown issue rainSTORM localization took 11% longer on an i7-6700K CPU.