Modalities

• Fluorescence wide-field microscope
• Super-resolution localization-based microscope system
• Differential Interference (DIC) microscopy
• Phase contrast
• OptoSplit two-colour and polarization sensitive imaging system
• Confocal fluorescence microscope
• Fluorescence lifetime (FLIM) confocal microscope
• Anisotropy confocal microscope
• Fluorescence correlation (FCS) confocal microscope

Software

• NIS Elements AR (Nikon): controls the Nikon microscope frame, laser unit, cameras, C2+ confocal.
• SymPhoTime64 (PicoQuant): controls the FLIM, FCS, anisotropy unit.
• Mathworks Matlab 2018a-2019a: simulation, analysis
• NI LabView 2017: equipment control

Key components

Nikon Eclipse Ti-E inverted microscope frame with:

• Perfect Focus System (keeps the sample in focus reducing axial drift)
• Motorized x-y stage (controlled via NIS software)
• Motorized filter turret with set:
  • 405/488/561/632;
  • 405/488/532/632;
  • 450LP
  • 640LP
  • DIC analyser
• Motorized filter wheel:
  • 690/70
  • 600/37
  • 582/75
  • 520/44
  • 445/40
• Piezo Z stage (closed loop 100 micron axial range)
• Immersion objectives: 100x (TIRF, NA=1.49), 60x (PLAN, NA=1.4),
• Nikon laser unit (AOTF controlled 405, 488, 561, and 647 nm lasers)
• Additional lasers (450, 532, 640 nm)
• EMCCD camera (Andor iXon 897)
• CMOS camera (Andor Zyla 4.2)
• Nikon DS-Fi3 (colour, 2880×2048 pixels)
• OptoSplit II+Bypass dual-view unit
• C2 confocal

PicoQuant LSM Upgrade kit:

• FLIM, FCS, anisotropy
• Laser Unit (exc.: 405, 480, 560 and 640 nm)
• 2*Hybrid detector