Equipment

Modalities

Software

Key components

Nikon Eclipse Ti-E inverted microscope frame with:

  • Perfect Focus System (keeps the sample in focus reducing axial drift)
  • Motorized x-y stage (controlled via NIS software)
  • Motorized filter turret with set:
    • 405/488/561/632;
    • 405/488/532/632;
    • 450LP
    • 640LP
    • DIC analyser
  • Motorized filter wheel:
    • 690/70
    • 600/37
    • 582/75
    • 520/44
    • 445/40
  • Piezo Z stage (closed loop 100 micron axial range)
  • Immersion objectives: 100x (TIRF, NA=1.49), 60x (PLAN, NA=1.4),
  • Nikon laser unit (AOTF controlled 405, 488, 561, and 647 nm lasers)
  • Additional lasers (450, 532, 640 nm)
  • EMCCD camera (Andor iXon 897)
  • CMOS camera (Andor Zyla 4.2)
  • Nikon DS-Fi3 (colour, 2880×2048 pixels)
  • OptoSplit II+Bypass dual-view unit
  • C2 confocal

PicoQuant LSM Upgrade kit:

  • FLIM, FCS, anisotropy
  • Laser Unit (exc.: 405, 480, 560 and 640 nm)
  • 2*Hybrid detector