Miklós Erdélyi, József Sinkó, Tamás Gajdos and Tibor Novák – “Enhanced simulator software for image validation and interpretation for multimodal localization super-resolution fluorescence microscopy “, Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 100710X (February 21, 2017); doi:10.1117/12.2250116; http://dx.doi.org/10.1117/12.2250116
A practical method has been presented for polarization sensitive localization based super-resolution microscopy using a birefringent dual wedge. The measurement of the polarization degree at single molecule level can reveal the chemical and physical properties of the local environment of the fluorescent dye molecule and can hence provide information about the sub-diffraction sized structure of biological samples. Polarization sensitive STORM imaging of the F-actins proved correlation between the orientation of fluorescent dipoles and the axis of the fibril.
On the Open Day of the Institute of Physics we spoke about the basics of magnification and resolution for elementary and high school students.
