A practical method has been presented for polarization sensitive localization based super-resolution microscopy using a birefringent dual wedge. The measurement of the polarization degree at single molecule level can reveal the chemical and physical properties of the local environment of the fluorescent dye molecule and can hence provide information about the sub-diffraction sized structure of biological samples. Polarization sensitive STORM imaging of the F-actins proved correlation between the orientation of fluorescent dipoles and the axis of the fibril.
On the Open Day of the Institute of Physics we spoke about the basics of magnification and resolution for elementary and high school students.
Public Lectures: Dr. Erdélyi Mikós
- 2016. 09. 26. 12:00: Localization optical microscopy
- 2016. 09. 26. 13:00: Elements of optical imaging
Location: SZTE TTIK Béke épület, Biofizika tanterem (6720 Szeged, Rerrich Béla tér 1. 3. emelet 310.).
We participated in the SMLM software challenge with our software. Also we developed two new 3D modalities (Astigmatic and Bi-Plane) for rainSTORM in a short time to compete in those categories as well. These are not released into the wild yet, but be patient.
- P016 – Localization analysis with rainSTORM – Tamás Gajdos, József Németh, József Sinkó, Dániel Varga, Eric J. Rees, Gábor Szabó, Miklós Erdélyi
- P039 – From measurement planning to the final image: software solutions for SMLM image processing – József Sinkó, Tamás Gajdos, Tibor Novák, József Németh, Eric J. Rees, Gábor Szabó, Miklós Erdélyi
The results can be found here, and our conclusion:
We are attending the ELMI2016 Meeting in Debrecen with two posters about the tools we use daily in localization microscopy:
- P007 – Localization analysis with rainSTORM – Tamás Gajdos, József Németh, József Sinkó, Dániel Varga, Eric J. Rees, Gábor Szabó, Miklós Erdélyi
- P041 – Artifact analysis in localization based microscopy – Dániel Varga, József Sinkó, Tamás Gajdos, Gábor Szabó, Miklós Erdélyi
If you have any question or you seek cooperation, don’t hesitate to contact us.
Location: Ketskeméty room, Bolyai Building 2nd floor
Date: 2016-APR-26. (Tue) 14:00
A figure was selected for the cover page from the article Manetsberger, J., Manton, J. D., Erdelyi, M. J., Lin, H., Rees, D., Christie, G., & Rees, E. J. (2015). Ellipsoid Localization Microscopy Infers the Size and Order of Protein Layers in Bacillus Spore Coats. Biophysical Journal, 109(10), 2058-2066.
Two years after rainSTORM v2.37 was released it was time for some refreshing. The rainSTORM v3.0 is here! It was tested under Matlab R2014a but it might run on older versions. (The user guide is still ‘in progress’ but the old one can help your journey.)
To start the application you have to extract the dowloaded file, change the working directory to rainSTORM and run this command: startup; rainSTORM;
Here are the highlights:
Our college, József Sinkó has successfully finished the Physics PhD program at the University of Szeged. His dissertation was written under the title „Studying and correcting the optical aberrations and reconstruction errors in localization based super-resolution microscopy” and was defended with a result of 100% on May 5th at the public defence.